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1 .GTX30605 Blimp1 antibody

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Blimp1 antibody


  • Host / ClonalityMouse Monoclonal
  • Clone Name3H2-E8
  • ApplicationsFACS, ICC/IF, IHC, WB
  • Species reactivityHuman, Mouse
Cat No: GTX30605
Package Price Qty
100 μlUSD 319.00
Add to cart
 
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
  • Anti-Blimp1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30605
  • Anti-Blimp1 antibody used in Western Blot (WB). GTX30605
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  • Datasheet
  • Application Information: Blimp1 antibody

Catalog Number GTX30605
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Product Name Blimp1 antibody
See all Blimp1 products
ApplicationsFACS, ICC/IF, IHC, WB
Application NoteRecognizes a band ~98 kD and may recognize one ~80 kDa (the beta form). Though this production of antibody has not been tested, previous lots have been used for immunohistochemistry, immunocytochemistry, and immunofluorescence on formalin-fixed paraffin-embedded or frozen sections. Antigen retrieval is recommended (EDTA buffer, microwave). This antibody demonstrates nuclear staining. This antibody does not immunoprecipitate.
Suggested working dilutions: * Western Blot - 1:1,000-1:5,000 Immunocytochemistry - (+) previous supernatant tested at 1:50-1:100 Immunofluorescence - (+) previous supernatant tested at 1:200 with tyramide amplification Immunohistochemistry - (+) previous supernatant tested at 1:200 with tyramide amplification *The investigator should determine the optimal working dilution for a specific application. *18-81 murine pre-B cells may be used as a negative control, in Western Blot, for this antibody.
Positive ControlsP3X (murine plasmocytoma) cell lysates
Form SuppliedLiquid
Concentration1.2 mg/ml (Please refer to the vial label for the specific concentration)

  • Specifications: Blimp1 antibody

BackgroundBlimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
Cellular LocalizationNuclear
SpecificityThis antibody is specific for the Blimp-1.
HostMouse
ClonalityMonoclonal
Clone Name3H2-E8
IsotypeIgG1
ImmunogenA fragment of the mouse Blimp-1 (residues 199-409).
Species ReactivityHuman, Mouse
ConjugationUnconjugated

  • Storage Conditions: Blimp1 antibody

Storage Buffer0.025 ml of Protein G purified mouse ascites [1.2 mg/ml] in PBS containing 0.1% Sodium Azide.
Storage InstructionStore at 4 degrees C. Long term (> 6 months) aliquot and store at -20 degrees C. Avoid repeated freeze thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Blimp1 antibody validated data
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - Blimp-1 expression by CD19+ (thick black line) or CD8+ 
      Top
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells
      Top
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice
      Top
  • Anti-Blimp1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30605
    • GTX30605 ICC/IF Image
      Immunocytochemistry/Immunofluorescence: Blimp-1 Antibody (3H2-E8) [GTX30605] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled
      Top
  • Anti-Blimp1 antibody used in Western Blot (WB). GTX30605
    • GTX30605 WB Image
      Western Blot: Blimp-1 Antibody (3H2-E8) [GTX30605] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using GTX30605. 1881: murine pre-B cell lysate (negative control)
      Top
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - Blimp-1 expression by CD19+ (thick black line) or CD8+ 
      Top
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells
      Top
  • Anti-Blimp1 antibody used in Flow cytometry/FACS. GTX30605
    • GTX30605 FACS Image
      Flow Cytometry: Blimp-1 Antibody (3H2-E8) [GTX30605] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice
      Top
  • Anti-Blimp1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30605
    • GTX30605 ICC/IF Image
      Immunocytochemistry/Immunofluorescence: Blimp-1 Antibody (3H2-E8) [GTX30605] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled
      Top
  • Anti-Blimp1 antibody used in Western Blot (WB). GTX30605
    • GTX30605 WB Image
      Western Blot: Blimp-1 Antibody (3H2-E8) [GTX30605] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using GTX30605. 1881: murine pre-B cell lysate (negative control)
      Top
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