|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||37 kDa (note)
|Positive Controls||H1299 , THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||BMI1 polycomb ring finger oncogene|
|Product Description||Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)|
|Background||Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. |
|Synonyms||FLVI2/BMI1 antibody, MGC12685 antibody, PCGF4 antibody, RNF51 antibody, BMI1 antibody, polycomb group protein Bmi1 antibody, ring finger protein 51 antibody, flvi-2/bmi-1 antibody, polycomb complex protein BMI-1 antibody, B lymphoma Mo-MLV insertion region 1 homolog antibody, murine leukemia viral (bmi-1) oncogene homolog antibody, polycomb group RING finger protein 4 antibody, BMI1 polycomb ring finger oncogene antibody|
|Cellular Localization||Nucleus , Cytoplasm|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (93%), Xenopus laevis (90%), Dog (99%), Cat (99%), Pig (100%), Chicken (97%), Chimpanzee (99%), Bovine (99%)|