|Applications||ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
| Immunohistochemistry||Assay-dependent dilution
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||47 kDa (note)
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||POU class 3 homeobox 2|
|Product Description||Rabbit Polyclonal antibody to Brn2 (POU class 3 homeobox 2)|
|Background||POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]|
|Synonyms||BRN2 antibody, OTF7 antibody, POUF3 antibody, POU3F2 antibody, brain-2 antibody, brain-specific homeobox/POU domain protein 2 antibody, nervous system-specific octamer-binding transcription factor N-Oct-3 antibody, octamer-binding protein 7 antibody, octamer-binding transcription factor 7 antibody, POU domain, class 3, transcription factor 2 antibody, POU class 3 homeobox 2 antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Chimpanzee, Bovine|
|Predict Reactivity Note||Xenopus laevis (97%), Chimpanzee (100%), Bovine (100%)|