C4d antibody [10-11]
|Applications||ELISA, ICC/IF, IHC-Fr, IHC-P, WB|
|IHC (Frozen sections)||1/100-1/750*
| IHC (Formalin-fixed paraffin-embedded sections)||Assay-dependent dilution
| Western blot||Assay-dependent dilution
IHC (Formalin-fixed paraffin-embedded sections) : It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomerμli and in some cases the peritubμlar capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffinNot tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Full Name||complement component 4B (Chido blood group)|
|Product Description||Mouse monoclonal antibody [10-11] to C4d|
|Background||This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]|
|Synonyms||C4B1 Antibody , CO4 Antibody , C4B2 Antibody , C4BD Antibody , C4B3 Antibody , C4B Antibody , complement component 4B (Chido blood group) Antibody , CPAMD3 Antibody , CH Antibody , C4B5 Antibody , C4F Antibody , C4B12 Antibody|
|Immunogen||Native C4d, from human plasma|