Carbonic Anhydrase IX antibody [GT12]

Name: Carbonic Anhydrase IX antibody [GT12]
Brand: GeneTex
SKU: GTX70020
Price: 419.00 USD

Cat. No. GTX70020

GTX70020 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX70020 IHC-P Image

Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

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GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

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GTX70020 WB Image

Sample (30 μg HeLa whole cell lysate)

A: 24 hr Untreated

B: 24 hr treatment with 100μM CoCl₂

C: 24 hr treatment with 200μM CoCl₂

D: 48 hr Untreated

E: 48 hr treatment with 100μM CoCl₂

F: 48 hr treatment with 200μM CoCl₂

Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX70020 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl₂ for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

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GTX70020 FCM Image

Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.

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GTX70020 IHC-P Image

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GTX70020 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25420025

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GTX70020 WB Image

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

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GTX70020 WB Image

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

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GTX70020 IHC-P Image

The data was published in the journal PLoS One in 2015. PMID: 25738958

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This Carbonic Anhydrase IX antibody [GT12] (GTX70020) is a mouse monoclonal antibody validated for detection of Carbonic Anhydrase IX by WB, ICC/IF, IHC-P, IHC-Fr, FCM, IP, and IHC. Reactivity has been shown in Human. This product is cited in 12 published studies.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT12
Isotype

IgG2b
Applications

WB ICC/IF IHC-P IHC-Fr FCM IP IHC
Reactivity

Human

Summary

Carbonic Anhydrase IX antibody (CA9 antibody) detects carbonic anhydrase 9, a ~50 kDa transmembrane glycoprotein. CA9 expression is enhanced by HIF1-alpha signaling in various biological processes, including cell proliferation and transformation. Found in many tissues, CA9 overexpression promotes tumor growth in various cancers.