CAIX antibody [GT12]
|Full Name||carbonic anhydrase IX|
|Synonyms||Carbonic anhydrase9, carbonic anhydrase IX, CA9, CA IX, CAIX, MN, CA 9, G250 antigen, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase 9, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250, RCC associated antigen G250, RCC associated protein G250, RCCassociated antigen G250, RCCassociated protein G250ic anhydrase IX, CA IX antibody, CAIX antibody, CA9 antibody, CA 9 antibody|
|Product Description||Mouse monocloncal antibody [GT12] to CAIX|
|Specificity||Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.|
|Background||Carbonic anhydrase IX (CA IX) is a member of the CA family of zinc-binding enzymes that catalyze a reversible conversion between carbon dioxide and carbonic acid, in a reaction that involves facilitated hydration of CO2 to H2CO3 followed by the spontaneous dissociation of H2CO3 into bicarbonate and proton. The CA IX molecule consists of a large extracellular domain (ECD), single-pass transmembrane region (TM) and a short intracytoplasmic (IC) tail. The ECD contains an N-terminally located PG-like region (which is absent from the other carbonic anhydrase isoforms) and a centrally located, well conserved catalytic domain (CA). CA IX is a cell surface protein that is present in human tumors, but not in the corresponding normal tissues. Moreover, expression of CA IX correlates with poor prognosis in many tumor types. CA IX plays a role in two phenomena involved in development of tumor phenotype - control of cell adhesion and pH regulation. Tight association of CA IX with tumors is to a major part related to tumor hypoxia.|
|Target||CAIX / CA9|
|Applications||FACS, ICC/IF, IHC, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||*
*Optimal dilutions/concentrations should be determined by the researcher.
|Storage Buffer||Aqueous buffered solution containing 1% BSA and ≤ 0.05% sodium azide|
|Storage Instruction||Aliquot and store undiluted at -20°C. Avoid repeated freeze/thaw cycles.|
|Notes||For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption. |