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  • CARD12 antibody [GT1387]

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1 .GTX631645 CARD12 antibody [GT1387]

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CARD12 antibody [GT1387]


  • Host / ClonalityMouse Monoclonal
  • Clone NameGT1387
  • ApplicationsWB
  • Species reactivityHuman
Cat No: GTX631645
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-CARD12 antibody [GT1387] used in Western Blot (WB). GTX631645
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  • Application Information: CARD12 antibody [GT1387]

Catalog Number GTX631645
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Product Name CARD12 antibody [GT1387]
See all CARD12 products
ApplicationsWB
Application Note
Suggested dilutionReference
Western Blot (WB)1:1000-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size116 kDa (note) IsoForm (3)
Positive Controlshuman NLRC4-transfected 293T
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified by Protein A.

  • Specifications: CARD12 antibody [GT1387]

Full NameNLR family, CARD domain containing 4
BackgroundIn C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]
SynonymsCLAN1 Antibody , CLANB Antibody , CLANA Antibody , IPAF Antibody , CLAN Antibody , CLR2.1 Antibody , CLANC Antibody , NLR family, CARD domain containing 4 Antibody , CLAND Antibody , NLRC4 Antibody
Cellular LocalizationCytoplasm
HostMouse
ClonalityMonoclonal
Clone NameGT1387
IsotypeIgG2a
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Bovine
Predict Reactivity NoteBovine (82%)
ConjugationUnconjugated

  • Storage Conditions: CARD12 antibody [GT1387]

Storage BufferPBS
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CARD12 antibody [GT1387] validated data
  • Anti-CARD12 antibody [GT1387] used in Western Blot (WB). GTX631645
    • GTX631645 WB Image
      CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody (GTX631645) diluted by 1:5000.
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  • Anti-CARD12 antibody [GT1387] used in Western Blot (WB). GTX631645
    • GTX631645 WB Image
      CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody (GTX631645) diluted by 1:5000.
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNLRC458484Q9NPP4, B2RBQ3606831NM_001199138NP_001186067116.18
Homo sapiensNLRC458484Q9NPP4, B2RBQ3606831NM_001199139NP_001186068116.18
Homo sapiensNLRC458484Q9NPP4, B2RBQ3606831NM_021209NP_067032116.18
    *MSDS
    MSDS-PBS.pdf
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All products are for research use only—Not for use in diagnostic procedures.