CBFb antibody [N1C3]
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||22 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||core-binding factor, beta subunit|
|Product Description||Rabbit polyclonal antibody to v (core-binding factor, beta subunit)|
|Background||The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||PEBP2B antibody, CBFB antibody, PEA2-beta antibody, core-binding factor subunit beta antibody, CBF-beta antibody, SL3-3 enhancer factor 1 subunit beta antibody, PEBP2-beta antibody, polyomavirus enhancer-binding protein 2 beta subunit antibody, "polyomavirus enhancer binding protein 2, beta subunit antibody", SL3-3 enhancer factor 1 beta subunit antibody, SL3/AKV core-binding factor beta subunit antibody, "core-binding factor, beta subunit antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human CBFb. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Zebrafish, Cat, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (98%), Rat (98%), Zebrafish (85%), Cat (100%), Chicken (96%), Rhesus Monkey (90%), Bovine (99%)|