CD151 antibody [11G5a]
|Applications||ELISA, FACS, IHC-Fr, IHC-P, IP, WB|
| ELISA||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1/100*
| Immunoprecipitation||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western blot : This antibody recognises human CD151 under non-reducing conditions.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|
|Full Name||CD151 molecule (Raph blood group)|
|Product Description||Mouse monoclonal antibody [11G5a] to CD151|
|Background||The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]|
|Synonyms||MER2 Antibody , GP27 Antibody , SFA1 Antibody , PETA-3 Antibody , RAPH Antibody , TSPAN24 Antibody|