CD2 antibody [LT2] (Low endotoxin, azide free)
|Applications||FACS, IHC-Fr, IP, Functional Assay|
|IHC (Frozen sections)||1/200-1/400*
| Immunoprecipitation||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Functional Assay : Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Full Name||CD2 molecule|
|Product Description||Mouse monoclonal antibody [LT2] to CD2|
|Background||CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]|
|Synonyms||CD2 molecule Antibody , LFA-2 Antibody , SRBC Antibody , T11 Antibody|
|Immunogen||Normal human peripheral blood lymphocytes.|
|Species Reactivity||Human, Chimpanzee, Rhesus Monkey, Cynomolgus monkey|