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Catalog Number
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GTX48118-PRO
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0
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Product Name
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CD252 Recombinant Protein (Active)
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| Full Name | tumor necrosis factor (ligand) superfamily, member 4 |
| Synonyms | TNFSF4, Gp34, CD252, OX40L |
| Background | OX40L, a member of the TNF superfamily of structurally related proteins, exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells (APCs), such as dendritic cells and activated B-cells, and also on various other cells such as vascular endothelial cells, mast cells, and natural killer cells. OX40L signals specifically through the OX40 receptor, which is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. OX40/OX40L functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. OX40/OX40L signaling plays an important role in immuno-regulatory communication, enabling the immune system to distinguish between “friend vs. foe” during activation; a mechanism typically termed immuno-tolerance. Recombinant OX40L is a glycosylated 133 amino acid protein corresponding to the extracellular TNF homologous domain of the full length transmembrane protein. It migrates with an apparent molecular mass of 15.5 – 25.0 kDa on SDS-PAGE. |
| Species | Human |
| Region/Sequence | QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL ILIHQNPGEF CVL |
| Expression System | High-5 Insect cells |
| Applications | ELISA, WB, Functional Assay |
| Application Note | Biological activity was determined by its ability to stimulate IL - 8 production by human PBMC using a concentration range of 30 - 100 ng/ml. Note: Results may vary with PBMC donors. |
| Conjugation | Unconjugated |
| Form Supplied | Lyophilized powder |
| Purity | Greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Concentration | Batch dependent
(Please refer to the vial label for the specific concentration) |
| Storage Instruction | The lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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