CD27 antibody

Host / ClonalityRabbit Polyclonal
ApplicationsIHC-P, WB
Species reactivityHuman

Cat No:

GTX104406

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: CD27 antibody

Catalog Number

GTX104406

Product Name

CD27 antibody

See all CD27 products

Applications

IHC-P, WB

Application Note

	Suggested dilution	Reference
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Western Blot (WB)	1:5000-1:20000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

29 kDa (note)

Positive Controls

K562

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: CD27 antibody

Full Name	CD27 molecule
Background	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq]
Synonyms	MGC20393 antibody, S152 antibody, T14 antibody, TNFRSF7 antibody, Tp55 antibody, CD27 antibody, T-cell activation antigen CD27 antibody, CD27 antigen antibody, tumor necrosis factor receptor superfamily member 7 antibody, T cell activation antigen S152 antibody, CD27L receptor antibody, "tumor necrosis factor receptor superfamily, member 7 antibody", T cell activation antigen CD27 antibody, CD27 molecule antibody
Cellular Localization	Membrane
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human CD27. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Conjugation	Unconjugated

Storage Conditions: CD27 antibody

Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Apoptosis > Anti-apoptosis Cell Biology > Apoptosis > Anti-apoptosis Immunology > CD marker More Hide

CD27 antibody validated data

GTX104406 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD27(GTX104406) antibody at 1:250 dilution.

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GTX104406 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX104406 diluted at 1:10000

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GTX104406 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD27(GTX104406) antibody at 1:250 dilution.

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GTX104406 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX104406 diluted at 1:10000

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Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	CD27	939	P26842	186711	NM_001242	NP_001233	29.16

*MSDS

MSDS-Glycerol.pdf

MSDS-Thimerosal.pdf

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CD27 antibody

CD27 antibody

Application Information: CD27 antibody

Specifications: CD27 antibody

Storage Conditions: CD27 antibody