|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||71 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , A375 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||CD2-associated protein|
|Product Description||Rabbit Polyclonal antibody to CD2AP (CD2-associated protein)|
|Background||This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq]|
|Synonyms||CMS antibody, DKFZp586H0519 antibody, CD2AP antibody, CD2-associated protein antibody, Cas ligand with multiple Src homology 3 (SH3) domains antibody, cas ligand with multiple SH3 domains antibody, adapter protein CMS antibody|
|Cellular Localization||Cytoplasm , cytoskeleton , Cell projection , ruffle |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (92%), Rhesus Monkey (96%), Bovine (90%)|