CD3 antibody [CD3-12]
|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, WB|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Frozen sections)||1/100*
|IHC (Formalin-fixed paraffin-embedded sections)||1/100*
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilization is required for this application.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification Note||From tissue culture supernatant|
|Full Name||CD3e molecule|
|Product Description||Rat monoclonal antibody [CD3-12] to CD3|
|Background||The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]|
|Synonyms||T3E Antibody , TCRE Antibody , CD3e molecule, epsilon (CD3-TCR complex) Antibody , CD3E Antibody|
|Specificity||This antibody raised against a peptide representing an invariant
cytoplasmic sequence within the CD3ε chain recognizes human CD3ε.|
|Immunogen||Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )|
|Species Reactivity||Human, Mouse, Chicken, Dog, Pig, Cat, Duck, Horse, Rabbit, Bovine, Rhesus Monkey, Cynomolgus monkey, Raccoon, Alpaca|