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CD31 antibody [MEC7.46]

Anti-CD31 antibody [MEC7.46] used in IHC (Frozen sections) (IHC-Fr). GTX54379

Cat. No. GTX54379

Reference 6

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MEC7.46

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Mouse
Package
100 μg ($399)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent

Note :

IHC-P
Serial paraffin-embedded 4 μm sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium.

IHC-Fr
Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent.

FACS
No fixation or permeabilization needed. Epitope is sensitive to trypsin!

IP
Lysate of 35S-Methionine labeled 5HV cells was used.
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Product Note

This antibody stains the extracellular domain of mouse PECAM-1.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_2887855

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet/endothelial cell adhesion molecule 1 , C85791 , Cd31 , PECAM-1 , Pecam

Cellular Localization

Cell membrane , Cell junction

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [MEC7.46] used in IHC (Frozen sections) (IHC-Fr). GTX54379

GTX54379 IHC-Fr Image

The data was published in the journal Sci Rep in 2018.PMID: 29463847

REFERENCE

Application Reference

Chen H et al. Regen Biomater 2023; 10 : rbac086 Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesis.
Zhao W et al. Bioeng Transl Med 2022; 7 (3) : e10303 Adaptive multi-degree-of-freedom in situ bioprinting robot for hair-follicle-inclusive skin repair: A preliminary study conducted in mice.
Nguyen HT et al. Sci Rep 2018; 8 (1) : 3326 Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.
Application : IHC-Fr
Reactivity : Mouse
Wilson R et al. Nat Commun 2016; 7 : 13597 MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment.
Reactivity : Mouse

Clone Reference

Wang C et al. PLoS One 2014; 9 (6):e100818 Local injection of * improves neovascularization in ischemic diabetic random flap by increasing HIF-1α and VEGF expression.
Application : IHC-P
Reactivity : Mouse
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Package List Price ($)
$ 399