CD33 antibody [WM53]
|Applications||FACS, IHC-Fr, IP, WB|
|IHC (Frozen sections)||1/50-1/100*
| Immunoprecipitation||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : Overnight incubation is recommended. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Immunoprecipitation : This antibody immunoprecipitates a protein of ~75kDa from myeloid cells, a smaller protein of approximately 67kDa has been observed in immunoprecipitates from lymphoid targets.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|