CD4 antibody [RPA-T4] (Low endotoxin, azide free)
|Applications||FACS, IHC-Fr, Functional Assay|
|IHC (Frozen sections)||1/200-1/2,000*
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.Not tested in other applications.
IHC (Frozen sections) : The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Functional Assay : This antibody has been reported to block gp120-CD4 interaction and inhibit syncytium formation.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|