PRODUCT
Summary
CD44 is an 82kDa multifunctional transmembrane glycoprotein involved in cell migration, proliferation, differentiation, and signaling pathways that mediate cell survival. It is involved in many malignancies as well as being recognized as a cancer stem cell marker. Importantly, it is expressed in leukocytes and is a marker of leukemia-initiating cells, being found to be critical in the pathogenesis of T cell acute lymphoblastic leukemia.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)
Product Note
IP/MS validation was supported by references (PMID:30377381)
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240596
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1
Cellular Localization
Membrane
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102111 WB Image
Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102111 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody (GTX102111) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX102111 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.
Reference: PMID: 10050880
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GTX102111 WB Image
Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.
Reference: PMID: 10050880
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GTX102111 IHC-P Image
Immunohistochemical analysis of paraffin-embedded mouse brain, using CD44(GTX102111) antibody at 1:500 dilution.
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GTX102111 ICC/IF Image
CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD44 stained by CD44 antibody (GTX102111) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX102111 IHC-P Image
CD44 antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma .
CD44 antibody (GTX102111) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102111 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44(GTX102111) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX102111 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102111 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(GTX102111) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102111 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX102111 IHC-P Image
CD44 antibody detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.CD44 stained by CD44 antibody (GTX102111) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102111 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX102111 WB Image
The data was published in the journal Cell Death Dis in 2015.PMID: 26136074
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GTX102111 WB Image
The data was published in the journal Cell Death Dis in 2015.PMID: 26136074
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GTX102111 WB Image
The data was published in the journal Cell Death Dis in 2015.PMID: 26136074
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GTX102111 WB Image
The data was published in the journal Sci Rep in 2018.PMID: 29955155
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GTX102111 LCI Image
The data was published in the 2021 in Commun Biol. PMID: 33637851
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GTX102111 ICC/IF Image
The data was published in the 2021 in Commun Biol. PMID: 33637851
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GTX102111 ICC/IF Image
The data was published in the journal Oncotarget in 2016. PMID: 27487131
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REFERENCE
REVIEW
CD44 antibody
Cat. No. GTX102111
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Rating
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( Average 4.5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MG63 cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Date :
Anonymous submitted on 23-Aug-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
SCM1 cells
Amount used :
75μg
Blocking :
5% skim milk, 37°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
