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  • CD45 antibody [GT0014]

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1 .GTX628507 CD45 antibody [GT0014]

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CD45 antibody [GT0014]

Biological & Orthogonal

  • Host / ClonalityMouse Monoclonal
  • Clone NameGT0014
  • ApplicationsFACS, ICC/IF, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 2 )
Cat No: GTX628507
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
  • Anti-CD45 antibody [GT0014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628507
  • Anti-CD45 antibody [GT0014] used in Western Blot (WB). GTX628507
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
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    Reference (2)
  • Bio-info
  • Protocol & MSDS
  • Application Information: CD45 antibody [GT0014]

Catalog Number GTX628507
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Publication ( 2 )
Product Name CD45 antibody [GT0014]
See all CD45 products
ApplicationsFACS, ICC/IF, WB
Application Note
Suggested dilutionReference
Flow cytometry/FACS1:1:50-1:200*  
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000* Y
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size147 kDa (note)
Positive ControlsRaji , Raji membrane extract
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified by Protein G.

  • Specifications: CD45 antibody [GT0014]

Full Nameprotein tyrosine phosphatase, receptor type, C
BackgroundThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]
SynonymsB220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, LCA antibody, LY5 antibody, T200 antibody, PTPRC antibody, CD45 antigen antibody, receptor-type tyrosine-protein phosphatase C antibody, protein tyrosine phosphatase, receptor type, c polypeptide antibody, T200 leukocyte common antigen antibody, leukocyte common antigen antibody, leukocyte-common antigen antibody, T200 glycoprotein antibody, protein tyrosine phosphatase, receptor type, C antibody
Cellular LocalizationMembrane
HostMouse
ClonalityMonoclonal
Clone NameGT0014
IsotypeIgG1
ImmunogenThe immunogen used to generate this antibody corresponds to human CD45
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
ConjugationUnconjugated

  • Storage Conditions: CD45 antibody [GT0014]

Storage BufferPBS
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Disease Related > Diabetes
  • Immunology > Adaptive Immunity > B cell > Development
  • Immunology > Adaptive Immunity > T cell > Development-Selection
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CD45 antibody [GT0014] validated data
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
      Sample: mouse splenocytes cell.
      Left: Unlabelled sample was used as a control.
      Right: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
      Acquisition of 40,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
      Top
  • Anti-CD45 antibody [GT0014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628507
    • GTX628507 ICC/IF Image
      CD45 antibody [GT0014] detects CD45 protein at cell membrane by immunofluorescent analysis.
      Sample: Raji cells were fixed in ice-cold MeOH for 5 min.
      Green: CD45 protein stained by CD45 antibody [GT0014] (GTX628507) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-CD45 antibody [GT0014] used in Western Blot (WB). GTX628507
    • GTX628507 WB Image  Biological & Orthogonal
      Raji whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.
      The observed M.W. is based on the publication: PMID: 24405902
      Top
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
      Sample: THP-1 cell.
      Black: Unlabelled sample was used as a control.
      Red: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
      Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
      Top
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45R antibody detects CD45R protein by flow cytometry analysis.
      Sample: THP-1 cell fixed in 4% paraformaldehyde at 4ºC for 15 min.
      Black: Isotype control (ab97239) dilution: 1:100.
      Green: CD45R antibody (GTX628507) dilution: 1:100.
      Top
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
      Sample: mouse splenocytes cell.
      Left: Unlabelled sample was used as a control.
      Right: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
      Acquisition of 40,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
      Top
  • Anti-CD45 antibody [GT0014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628507
    • GTX628507 ICC/IF Image
      CD45 antibody [GT0014] detects CD45 protein at cell membrane by immunofluorescent analysis.
      Sample: Raji cells were fixed in ice-cold MeOH for 5 min.
      Green: CD45 protein stained by CD45 antibody [GT0014] (GTX628507) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-CD45 antibody [GT0014] used in Western Blot (WB). GTX628507
    • GTX628507 WB Image  Biological & Orthogonal
      Raji whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.
      The observed M.W. is based on the publication: PMID: 24405902
      Top
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
      Sample: THP-1 cell.
      Black: Unlabelled sample was used as a control.
      Red: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
      Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
      Top
  • Anti-CD45 antibody [GT0014] used in Flow cytometry/FACS. GTX628507
    • GTX628507 FACS Image
      CD45R antibody detects CD45R protein by flow cytometry analysis.
      Sample: THP-1 cell fixed in 4% paraformaldehyde at 4ºC for 15 min.
      Black: Isotype control (ab97239) dilution: 1:100.
      Green: CD45R antibody (GTX628507) dilution: 1:100.
      Top
Back to Top
  • Reference for CD45 antibody [GT0014]
Application References Application Reactivity
Fernando MR et al. New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes. PLoS One 12 (8):e0183915 (2017). WB Human
Yang J et al. Adipose-derived stem cells from the breast. J Res Med Sci 19 (2):112-6 (2014). ICC/IF Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPTPRC5788Q6PJK7, Q0VAE8, P08575126200, 151460, 608971, 609532
    *MSDS
    MSDS-PBS.pdf
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