CD47 antibody [BRIC126]
|Applications||FACS, IHC-Fr, IP, WB, Functional Assay|
| IHC (Frozen sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
| Western blot||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
Western blot : This antibody recognizes CD47 under non-reducing conditions, the CD47 epitoperecognized is lost on reduction.
Functional Assay : This product contains sodium azide, removal by dialysis is recommended prior touse in functional assays.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||CD47 molecule|
|Product Description||Mouse monoclonal antibody [BRIC126] to CD47|
|Background||This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]|
|Synonyms||MER6 Antibody , OA3 Antibody , IAP Antibody , CD47 molecule Antibody|
|Species Reactivity||Human, Dog, Pig, Sheep, Bovine|