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  • CD59 antibody [MEM-43] (Azide free)

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1 .GTX74620 CD59 antibody [MEM-43] (Azide free)

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CD59 antibody [MEM-43] (Azide free)


  • Host / ClonalityMouse Monoclonal
  • Clone NameMEM-43
  • ApplicationsELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
  • Species reactivityHuman
Cat No: GTX74620
Package Price Qty
500 μgUSD 409.00
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  • Anti-CD59 antibody [MEM-43] (Azide free) used in Flow cytometry/FACS. GTX74620
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  • Application Information: CD59 antibody [MEM-43] (Azide free)

Catalog Number GTX74620
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Product Name CD59 antibody [MEM-43] (Azide free)
See all CD59 products
ApplicationsELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Application Note
Suggested dilutionReference
ELISAAssay-dependent dilution  
Flow cytometry/FACS1/5-1/10*  
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution  
IHC (Frozen sections)Assay-dependent dilution  
IHC (Formalin-fixed paraffin-embedded sections)Assay-dependent dilution  
ImmunoprecipitationAssay-dependent dilution  
Western Blot (WB)Assay-dependent dilution  
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blot (WB) : This product recognizes CD59 under non-reducing conditions.
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationProtein A purified
Purification NoteFrom tissue culture supernatant

  • Specifications: CD59 antibody [MEM-43] (Azide free)

Full NameCD59 molecule
BackgroundThis gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
SynonymsMACIF Antibody , 1F5 Antibody , MIN2 Antibody , MIN3 Antibody , HRF-20 Antibody , 16.3A5 Antibody , MSK21 Antibody , EJ16 Antibody , HRF20 Antibody , EL32 Antibody , CD59 molecule, complement regulatory protein Antibody , G344 Antibody , MIC11 Antibody , MIN1 Antibody , MEM43 Antibody , MAC-IP Antibody , MIRL Antibody , EJ30 Antibody , p18-20 Antibody
HostMouse
ClonalityMonoclonal
Clone NameMEM-43
IsotypeIgG2a
ImmunogenThymocytes and T lymphocytes.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: CD59 antibody [MEM-43] (Azide free)

Storage BufferPBS
Storage InstructionKeep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. This product may contain precipitation. Recommend microcentrifugation before use.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Immunology > CD marker
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CD59 antibody [MEM-43] (Azide free) validated data
  • Anti-CD59 antibody [MEM-43] (Azide free) used in Flow cytometry/FACS. GTX74620
    • GTX74620 FACS Image
      FACS analysis of human peripheral blood lymphocytes using GTX74620 CD59 antibody [MEM-43] (Azide free).
      Top
  • Anti-CD59 antibody [MEM-43] (Azide free) used in Flow cytometry/FACS. GTX74620
    • GTX74620 FACS Image
      FACS analysis of human peripheral blood lymphocytes using GTX74620 CD59 antibody [MEM-43] (Azide free).
      Top
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    *MSDS
    MSDS-PBS.pdf
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