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CD66e antibody

Anti-CD66e antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108732
Anti-CD66e antibody used in IHC (Paraffin sections) (IHC-P). GTX108732
Anti-CD66e antibody used in Western Blot (WB). GTX108732

Cat. No. GTX108732

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD66e products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD66e. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carcinoembryonic antigen related cell adhesion molecule 5 , CD66e , CEA

Cellular Localization

Cell membrane , GPI-anchor

Background

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CD66e antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108732

GTX108732 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD66e(GTX108732) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-CD66e antibody used in IHC (Paraffin sections) (IHC-P). GTX108732

GTX108732 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using CD66e(GTX108732) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD66e antibody used in Western Blot (WB). GTX108732

GTX108732 WB Image

CD66e antibody detects CD66e protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD66e antibody (GTX108732) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Allen A Smith et al. Intractable Rare Dis Res 2021; 10 (1) : 58-59 Pre-Paget cells express a Paget cell marker before losing a keratinocyte marker.
Application : IHC-P
Reactivity : Human
Smith AA et al. Intractable Rare Dis Res 2019; 8 (3) : 203-205 Pre-Paget cells: Evidence of keratinocyte origin of extramammary Paget's disease.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

CD66e antibody Cat. No. GTX108732

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CD66e antibody used in IHC (Paraffin sections) (IHC-P). GTX108732
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169