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  • CD71 antibody [MEM-75] (PE)

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1 .GTX18242 CD71 antibody [MEM-75] (PE)

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CD71 antibody [MEM-75] (PE)


  • Host / ClonalityMouse Monoclonal
  • Clone NameMEM-75
  • ApplicationsFACS, ICC/IF
  • Species reactivityHuman
  • Publication  ( 1 )
Cat No: GTX18242
Package Price Qty
100 testUSD 319.00
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  • Anti-CD71 antibody [MEM-75] (PE) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18242
  • Anti-CD71 antibody [MEM-75] (PE) used in Flow cytometry/FACS. GTX18242
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  • Application Information: CD71 antibody [MEM-75] (PE)

Catalog Number GTX18242
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Publication ( 1 )
Product Name CD71 antibody [MEM-75] (PE)
See all CD71 products
ApplicationsFACS, ICC/IF
Application NoteFACS: Use at a concentration of 20 μl per 100 μl of whole human blood. Optimal dilutions/concentrations should be determined by the end user.
Positive ControlsHuman blood cells
Form SuppliedLiquid
Purification NoteThe conjugate wass purified by size exclusion chromatography.

  • Specifications: CD71 antibody [MEM-75] (PE)

Full Nametransferrin receptor (p90, CD71)
BackgroundCellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.The transferrin receptor has been structurally characterized as a sulfide-bound dimer of identical glycoprotein subunits of 95 kD. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines.
SynonymsTFR, TFR1, 7037, 190010, Transferrin receptor, TFRC, CD71, P02786, TRFR
Cellular LocalizationType II membrane protein. Secreted.
SpecificityCD71 antigen (transferrin receptor). This antibody does not block binding of transferrin to the receptor.
HostMouse
ClonalityMonoclonal
Clone NameMEM-75
IsotypeIgG1
ImmunogenPre-B cell line NALM-6 (Human).
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationPhycoerythrin (PE)

  • Storage Conditions: CD71 antibody [MEM-75] (PE)

Storage BufferPreservative: 15mM Sodium Azide. Constituents: PBS, 0.2% BSA (protease-free). pH 7.4
Storage InstructionKeep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Hypoxia
  • Cancer > Tumor biomarkers
  • Cancer > Type of cancer > Breast > Other
  • Cancer > Type of cancer > Liver
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CD71 antibody [MEM-75] (PE) validated data
  • Anti-CD71 antibody [MEM-75] (PE) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18242
    • GTX18242 ICC/IF Image
      Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (GTX29179; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue)
      Top
  • Anti-CD71 antibody [MEM-75] (PE) used in Flow cytometry/FACS. GTX18242
    • GTX18242 FACS Image
      Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 [MEM-75]: Biotin (GTX78311); detection by Streptavidin-PE. Total viable cells were used for analysis
      Top
  • Anti-CD71 antibody [MEM-75] (PE) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18242
    • GTX18242 ICC/IF Image
      Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (GTX29179; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue)
      Top
  • Anti-CD71 antibody [MEM-75] (PE) used in Flow cytometry/FACS. GTX18242
    • GTX18242 FACS Image
      Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 [MEM-75]: Biotin (GTX78311); detection by Streptavidin-PE. Total viable cells were used for analysis
      Top
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  • Reference for CD71 antibody [MEM-75] (PE)
Clone References Application Reactivity
Schroeder N et al. The lipid raft-associated protein CD98 is required for vaccinia virus endocytosis. J Virol 86 (9):4868-82 (2012).
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