CD74 antibody [Bu45]
|Applications||FACS, ICC/IF, IHC-Fr, IP, Functional Assay|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilisation may enhance CD74 staining. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing.
Functional Assay : This product contains sodium azide, removal by dialysis is recommended prior touse in functional assays.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||CD74 molecule|
|Product Description||Mouse Monoclonal antibody [Bu45] to CD74|
|Background||The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]|
|Synonyms||CD74 molecule, major histocompatibility complex, class II invariant chain Antibody , Ia-GAMMA Antibody , DHLAG Antibody , HLADG Antibody , II Antibody|
|Immunogen||B lymphoblastoid cell line HFB1.|