CDC2 (phospho Tyr15) antibody
|Applications||IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||34 kDa (note)
|Positive Controls||HeLa (untreated) , HeLa (2mM double thymidine treatment and recovery for 3.5hr) , HeLa (2mM double thymidine treatment and recovery for 12hr) , HeLa (100ng/ml Nocodazole treatment for 24hr) , Rat-2 (untreated) , Rat-2 (100ng/ml Nocodazole treatment for 24|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||cyclin-dependent kinase 1|
|Product Description||Rabbit Polyclonal antibody to CDC2 (phospho Tyr15) (cyclin-dependent kinase 1)|
|Background||The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||CDC2 antibody, CDC28A antibody, DKFZp686L20222 antibody, MGC111195 antibody, P34CDC2 antibody, CDK1 antibody, p34 protein kinase antibody, cell division protein kinase 1 antibody, cell cycle controller CDC2 antibody, cell division cycle 2, G1 to S and G2 to M antibody, cell division control protein 2 homolog antibody, cyclin-dependent kinase 1 antibody|
|Cellular Localization||Nucleus |
|Species Reactivity||Human, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Zebrafish, Japanese medaka, Drosophila, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Mouse (100%), Zebrafish (100%), Japanese medaka (100%), Drosophila (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|