CHFR antibody, C-term
|Application Note||Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5µg/ml. This antibody is expected to recognise all reported isoforms.|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX13773-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||checkpoint with forkhead and ring finger domains|
|Product Description||Goat polyclonal antibody to CHFR|
|Background||A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.|
|Synonyms||E3 ubiquitin-protein ligase CHFR, RING finger protein 196, checkpoint with forkhead and ring finger domains, RNF116, CHFR, RNF196, FLJ10796, FLJ33629, E3 ubiquitinprotein ligase CHFR, RING finger protein196|
|Specificity||This antibody is specific for CHFR.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Pig, Bovine|