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CKII alpha antibody [N1C3]

Anti-CKII alpha antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110726
Anti-CKII alpha antibody [N1C3] used in Western Blot (WB). GTX110726
Anti-CKII alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110726

Cat. No. GTX110726

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKII alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950050

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

casein kinase 2 alpha 2 , CK2A2 , CK2alpha' , CSNK2A1

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Database

Research Area

DATA IMAGES

Anti-CKII alpha antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110726

GTX110726 IHC-P Image

CKII alpha antibody [N1C3] detects CKII alpha protein at cytoplasm in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
CKII alpha antibody [N1C3] (GTX110726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CKII alpha antibody [N1C3] used in Western Blot (WB). GTX110726

GTX110726 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX110726 diluted at 1:3000

Anti-CKII alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110726

GTX110726 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CKII alpha prime polypeptide(GTX110726) antibody at 1:500 dilution.

REFERENCE

There are currently no references for CKII alpha antibody [N1C3] (GTX110726). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CKII alpha antibody [N1C3] (GTX110726). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169