APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , HepG2 , 293T
Predict Reactivity
Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.21 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human COPD. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949678
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
archain 1 , COPD , SRMMD
Cellular Localization
Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa
Background
This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103252 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody (GTX103252) diluted at 1:2000.
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GTX103252 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD(GTX103252) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.
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GTX103252 WB Image
Sample(30 ug whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX103252 diluted at 1:500
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GTX103252 IHC-P Image
COPD antibody detects COPD protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.COPD stained by COPD antibody (GTX103252) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX103252 IHC-Fr Image
COPD antibody detects COPD protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse mouse cerebellum.Green: COPD stained by COPD antibody (GTX103252) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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REFERENCE
There are currently no references for COPD antibody (GTX103252). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COPD antibody (GTX103252). Be the first to share your experience with this product.
