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COX4 antibody [GT6310]

Anti-COX4 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628886
Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886
Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886
Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

Cat. No. GTX628886

Host

Mouse

Clonality

Monoclonal

Clone Name

GT6310

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , rat muscle

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888063

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628886

GTX628886 ICC/IF Image

COX4I1 antibody detects COX4I1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: COX4I1 protein stained by COX4I1 antibody (GTX628886) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis.
A. 50 μg rat muscle lysate/extract
12 % SDS-PAGE
COX4 antibody [GT6310] (GTX628886) dilution: 1:1000

Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.

Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COX4 stained by COX4 antibody [GT6310] (GTX628886) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations