|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||20 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||cytochrome c oxidase subunit IV isoform 1|
|Product Description||Rabbit Polyclonal antibody to COX4 (cytochrome c oxidase subunit IV isoform 1)|
|Background||Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]|
|Synonyms||COX4 antibody, COX4-1 antibody, COXIV antibody, FLJ23483 antibody, MGC72016 antibody, COX4I1 antibody, "cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody", COX IV-1 antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV isoform 1 antibody|
|Cellular Localization||Mitochondrion inner membrane|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Rhesus Monkey (92%), Chimpanzee (100%), Bovine (81%)|