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1 .GTX15917 CPEB1 antibody, C-term

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CPEB1 antibody, C-term


  • Host / ClonalityGoat Polyclonal
  • ApplicationsWB
  • Species reactivityHuman
Cat No: GTX15917
Package Price Qty
100 μgUSD 319.00
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  • Anti-CPEB1 antibody, C-term used in Western Blot (WB). GTX15917
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  • Datasheet
  • Application Information: CPEB1 antibody, C-term

Catalog Number GTX15917
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Product Name CPEB1 antibody, C-term
See all CPEB1 products
ApplicationsWB
Application NoteApprox 65-70kDa band observed in human brain lysates (predicted MW of 65kDa according to NP_085097). Recommended for use at 0.5-2µg/ml. Please note this band was always accompanied by a slightly stonger band at approx 48kDa and further both bands could be blocked by incubation with the immunising peptide. This antibody is expected to recognise alle reported isoforms. Variants (NP_001073002.1; NP_001073003.1) encode the same isoform.
Positive ControlsThe peptide used to product this antibody is available for purchase (Catalog Number:GTX15917-PEP).
Form SuppliedLiquid
Concentration0.5 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Specifications: CPEB1 antibody, C-term

Full Namecytoplasmic polyadenylation element binding protein 1
BackgroundThe regulated translation of messenger RNA is essential for cell-cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, which is one mechanism of controlling translation, is driven by CPEB (Cytoplasmic Polyadenylation Element Binding protein), a highly conserved, sequence specific RNA binding protein that binds to the cytoplasmic polyadenylation element, and modulates translational repression and mRNA localization. In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB. Early metazoan development is programmed by maternal mRNAs inherited by the egg at the time of fertilization. These mRNAs are not translated en masse at any one time or at any one place, but instead their expression is regulated both temporally and spatially. Mammalian neurons, particularly in the synapto dendritic compartment, also contain localized mRNAs such as that encoding alpha CaMKII. Here, synaptic activation drives local translation, an event that is involved in synaptic plasticity and possibly long term memory storage. Synaptic translation of alpha CaMKII mRNA also appears to be controlled by CPEB, which is enriched in the postsynaptic density. Therefore, CPEB controlled local translation may influence such seemingly disparate processes as the cell cycle and synaptic plasticity.
SynonymsCEBP, CPEbinding protein1, CPE-BP1, CPEbinding protein 1, MGC60106, cytoplasmic polyadenylation element binding protein1, cytoplasmic polyadenylation element binding protein 1, CPEB, FLJ13203, MGC34136, CPE-binding protein 1, CPEB1, CPEBP1, CPE BP1, CPE binding protein 1
Cellular LocalizationCytoplasmic
SpecificityThis antibody is specific for CPEB1.
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenC-PLMRNQKNRDSS
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Bovine
ConjugationUnconjugated

  • Storage Conditions: CPEB1 antibody, C-term

Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Instruction Aliquot and store at -20°C. Minimize freezing and thawing.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CPEB1 antibody, C-term validated data
  • Anti-CPEB1 antibody, C-term used in Western Blot (WB). GTX15917
    • GTX15917 WB Image
      GTX15917 staining (0.5µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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  • Anti-CPEB1 antibody, C-term used in Western Blot (WB). GTX15917
    • GTX15917 WB Image
      GTX15917 staining (0.5µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
      Top
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