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CREB antibody

KO/KD Comparison Orthogonal
Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Frozen sections) (IHC-Fr). GTX112846
Anti-CREB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112846
Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in Western Blot (WB). GTX112846

Cat. No. GTX112846

Reference 16
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat nuclear extract , 293T

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CREB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885324

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CREB antibody (GTX112846) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CREB antibody (GTX112846) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CREB antibody used in IHC (Frozen sections) (IHC-Fr). GTX112846

GTX112846 IHC-Fr Image

CREB antibody detects CREB protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: CREB protein stained by CREB antibody (GTX112846) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CREB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112846

GTX112846 ICC/IF Image

CREB antibody detects CREB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CREB protein stained by CREB antibody (GTX112846) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
CREB antibody (GTX112846) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:1000.

REFERENCE

Application Reference

Yu S et al. Aging (Albany NY) 2024; 16 (5) : 4348-4362 Prenatal diesel exhaust exposure alters hippocampal synaptic plasticity in offspring.
YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

CREB antibody Cat. No. GTX112846

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-CREB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112846
Date : Anonymous submitted on 23-Aug-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 20% BlockOne in TBST
Fixation : 4% PFA in PBS, 15 min
Permeabilization /Antigen retrieval : TBST
Primary Antibody : 1:1000, 25°C, 12Hr
Anti-CREB antibody used in Western Blot (WB). GTX112846
Date : Anonymous submitted on 23-Aug-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco-2 cells
Amount used : 20μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:4000, 25°C, 12Hr
Anti-CREB antibody used in Western Blot (WB). GTX112846
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC3 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM ADC treatment for 24h.
Anti-CREB antibody used in Western Blot (WB). GTX112846
Date : Anonymous submitted on 13-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CREB1-transfected 293T cells
Amount used : 1*10^5 cells
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1: 293T cells transfected with pCDNA3.1-myc-human CREB. Lane2: 293T cells transfected with pCDNA3.1.
Submit a Review
Application Reference
Yu S et al. Aging (Albany NY) 2024; 16 (5):4348-4362 Prenatal diesel exhaust exposure alters hippocampal synaptic plasticity in offspring.
YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
2021;:e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
Application : WB
Reactivity : Mouse
Hseu YC et al. Redox Biol 2021; 44:102007 The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.
Application : WB
Reactivity : Human
Wang YY et al. Cancers (Basel) 2020; 12 (12) Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.
Application : WB
Reactivity : Human
Cao K et al. Aging (Albany NY) 2020; 12 (2):1792-1807 The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors.
Torelli Hijo AH et al. Heliyon 2019; 5 (10):e02611 High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity.
Application : WB
Reactivity : Mouse
Andressa Harumi Torelli Hijo et al. Heliyon 2019; 5 (10):e02611 High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity.
Application : WB
Reactivity : Mouse
Ho SY et al. BMC Cancer 2018; 18 (1):480 Development of a computational promoter with highly efficient expression in tumors.
Application : WB
Reactivity : Human
Wu G et al. Epilepsy Research 2017; (Epub) PKC/CREB pathway mediates the expressions of GABAA receptor subunits in cultured hippocampal neurons after low-Mg2+ solution treatment.
Tsunematsu H et al. BMC Neurosci 2017; 18 (1):55 Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures.
Application : ICC/IF
Reactivity : Mouse
Chang CY et al. Biomed Res Int 2015; 2015:159015 Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169