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1 .GTX130379 CREB (phospho Ser133) antibody

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CREB (phospho Ser133) antibody

Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsWB
  • Species reactivityHuman, Mouse
Cat No: GTX130379
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
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  • Datasheet
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  • Application Information: CREB (phospho Ser133) antibody

Catalog Number GTX130379
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Product Name CREB (phospho Ser133) antibody
See all CREB products
ApplicationsWB
Application Note
Suggested dilutionReference
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size37 kDa (note) IsoForm (3)
Positive ControlsA431 (200 ng/ml EGF treatment for 30 min) , NIH-3T3 (5 ng/ml PDGF treatment for 15 min) , NIH-3T3 , NIH-3T3 (0.1 μg/ml hFGF2 treatment for 10 min)
Form SuppliedLiquid
Concentration0.39 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: CREB (phospho Ser133) antibody

Full NamecAMP responsive element binding protein 1
BackgroundThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
Cellular LocalizationNucleus
SpecificityThis antibody is raised against human CREB phosphorylated at Ser133, and it also may detect the phosphorylated ATF1 protein due to sequence homology.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human CREB (phospho Ser133). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: CREB (phospho Ser133) antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Prostate cancer
  • Disease Related > Alzheimer's disease
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Neuroscience > Circadian rhythm
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CREB (phospho Ser133) antibody validated data
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379
    • GTX130379 WB Image
      Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCREB11385P16220, Q53X93, Q5U0J5123810, 612160NP_00130772224.68
Homo sapiensCREB11385P16220, Q53X93, Q5U0J5123810, 612160NM_004379NP_00437035.15
Homo sapiensCREB11385P16220, Q53X93, Q5U0J5123810, 612160NM_134442NP_60439136.70

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusCreb112912Q543W0, Q62347, Q01147, Q547S9NM_009952NP_03408236.69
Mus musculusCreb112912Q543W0, Q62347, Q01147, Q547S9NM_001037726NP_00103281530.97
Rattus norvegicusCreb181646P15337NM_031017NP_11227936.65
Mus musculusCreb112912Q543W0, Q62347, Q01147, Q547S9NM_133828NP_59858935.13
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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