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1 .GTX100901 CRK antibody [N2C3]

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CRK antibody [N2C3]

Comparative Abs

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
Cat No: GTX100901
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
  • Anti-CRK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100901
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
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  • Datasheet
  • Bio-info
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  • Application Information: CRK antibody [N2C3]

Catalog Number GTX100901
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Product Name CRK antibody [N2C3]
See all CRK products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size34 kDa (note)
Positive Controls293T , A431 , HeLa , HepG2 , NIH-3T3
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: CRK antibody [N2C3]

Full Namev-crk sarcoma virus CT10 oncogene homolog (avian)
BackgroundThis gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]
SynonymsCRKII antibody, CRK antibody, adapter molecule crk antibody, proto-oncogene c-Crk antibody, v-crk sarcoma virus CT10 oncogene homolog (avian) antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Pig, Chicken, Bovine
Predict Reactivity NoteRat (99%), Xenopus laevis (87%), Pig (98%), Chicken (91%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: CRK antibody [N2C3]

Storage Buffer1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CRK antibody [N2C3] validated data
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
    • GTX100901 WB Image  Comparative Abs
      Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] (GTX100901) diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
      *The competitor is not affiliated with GeneTex and does not endorse this product.
      Top
  • Anti-CRK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100901
    • GTX100901 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using CRK(GTX100901) antibody at 1:200 dilution.
      Top
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
    • GTX100901 WB Image
      Sample (30 ug of whole cell lysate)
      A:NIH-3T3 12% SDS PAGE
      GTX100901 diluted at 1:1000
      Top
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
    • GTX100901 WB Image  Comparative Abs
      Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] (GTX100901) diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
      *The competitor is not affiliated with GeneTex and does not endorse this product.
      Top
  • Anti-CRK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100901
    • GTX100901 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using CRK(GTX100901) antibody at 1:200 dilution.
      Top
  • Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
    • GTX100901 WB Image
      Sample (30 ug of whole cell lysate)
      A:NIH-3T3 12% SDS PAGE
      GTX100901 diluted at 1:1000
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCRK1398B0LPE8, P46108, Q96GA9164762NM_016823NP_05843133.84

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusCrk12928Q64010, Q5ND51NM_133656NP_59841733.82
Rattus norvegicusCrk54245Q63768NM_019302NP_06217533.85
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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