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CXCR4 antibody

KO/KD
Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Flow cytometry (FACS). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

Cat. No. GTX22074

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all CXCR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 10 μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 0.1 μg/ml
IP Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

Product Note

CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence corresponding to amino acids 1-14 of human CD184.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_369470

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane,Cell junction,Lysosome

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074

GTX22074 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 2 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of various sample lysates using GTX22074 CXCR4 antibody.
Loading : 20μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074

GTX22074 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 20 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of various sample lysates using GTX22074 CXCR4 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-CXCR4 antibody used in Flow cytometry (FACS). GTX22074

GTX22074 FACS Image

FACS analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 0.1 μg/ml
Purple: cells without staining
Green: Isotype control
Red : CXCR4 antibody

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of CXCR4 partial recombinant protein using GTX22074 CXCR4 antibody.
Dilution : 0.1 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) using GTX22074 CXCR4 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22074

GTX22074 IHC-P Image

IHC-P analysis of human spleen tissue using GTX22074 CXCR4 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of HeLa cell lysate using GTX22074 CXCR4 antibody.
Working concentration : 0.5 μg/ml

REFERENCE

Application Reference

Chang TM et al. Int J Biol Sci 2023; 19 (5) : 1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : Neutralizing/Inhibition
Reactivity : Human
Min Leng et al. J Diabetes Res 2021; 2021 : 9962877 Experimental Study on the Effect of Allogeneic Endothelial Progenitor Cells on Wound Healing in Diabetic Mice.
Application : WB
Reactivity : Mouse
Shuhei Kamada et al. Front Oncol 2021; 11 : 758503 Clinicopathological and Preclinical Patient-Derived Model Studies Define High Expression of NRN1 as a Diagnostic and Therapeutic Target for Clear Cell Renal Cell Carcinoma.
Application : WB, IHC-P
Reactivity : Human
Kun-Ming C et al. Arch Med Res 2020; 51 (4) : 297-302 Potential Anticancer Effect of Celastrol on Hepatocellular Carcinoma by Suppressing CXCR4-related Signal and Impeding Tumor Growth in?Vivo.
Application : WB
Reactivity : Human
Zhao Z et al. Int J Cancer 2017; (Epub) Intraductal papillary mucinous neoplasm of the pancreas rapidly xenografts in chicken eggs and predicts aggressiveness.
Application : IHC-Fr
Reactivity : Human
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REVIEW

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Application Reference
Chang TM et al. Int J Biol Sci 2023; 19 (5):1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : Neutralizing/Inhibition
Reactivity : Human
Min Leng et al. J Diabetes Res 2021; 2021:9962877 Experimental Study on the Effect of Allogeneic Endothelial Progenitor Cells on Wound Healing in Diabetic Mice.
Application : WB
Reactivity : Mouse
Shuhei Kamada et al. Front Oncol 2021; 11:758503 Clinicopathological and Preclinical Patient-Derived Model Studies Define High Expression of NRN1 as a Diagnostic and Therapeutic Target for Clear Cell Renal Cell Carcinoma.
Application : WB, IHC-P
Reactivity : Human
Kun-Ming C et al. Arch Med Res 2020; 51 (4):297-302 Potential Anticancer Effect of Celastrol on Hepatocellular Carcinoma by Suppressing CXCR4-related Signal and Impeding Tumor Growth in?Vivo.
Application : WB
Reactivity : Human
Feng H et al. Br J Cancer 2017; (Epub) ALDH1A3 affects colon cancer in vitro proliferation and invasion depending on CXCR4 status.
Zhao Z et al. Int J Cancer 2017; (Epub) Intraductal papillary mucinous neoplasm of the pancreas rapidly xenografts in chicken eggs and predicts aggressiveness.
Application : IHC-Fr
Reactivity : Human
Zhang Y et al. Oncotarget 2015; 6 (12):9999-10015 aspirin counteracts cancer stem cell features, desmoplasia and * resistance in pancreatic cancer.
Application : IHC-Fr
Reactivity : Human
Isayev O et al. Oncotarget 2014; 5 (13):5177-89 inhibition of glucose turnover by 3-bromopyruvate counteracts pancreatic cancer stem cell features and sensitizes cells to *.
Application : IHC-Fr
Reactivity : Human
Jin UH et al. BMC Cancer 2014; 14:498 The aryl hydrocarbon receptor ligand omeprazole inhibits breast cancer cell invasion and metastasis.
Application : WB, IHC-P
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399