CaMK1 (phospho Thr177) antibody

Cat No:

GTX54970

Package	Price	Qty
100 μl	USD 319.00

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Catalog Number	GTX54970 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	CaMK1 (phospho Thr177) antibody See all CaMKI products
Applications	ICC/IF, IHC-P, WB
Application Note	WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Form Supplied	Liquid
Purification	The antibody was purified by immunogen affinity chromatography.

Full Name	calcium/calmodulin-dependent protein kinase I
Background	Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]
Synonyms	CAMKI Antibody , CAMK1 Antibody , calcium/calmodulin-dependent protein kinase I Antibody
Specificity	Recognizes endogenous levels of CaMK1 alpha (pT177) protein.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CaMK1 alpha (pT177). The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Conjugation	Unconjugated

Storage Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Instruction	Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Stem Cell Development > Development > Ectoderm > Nervous system development More Hide

CaMK1 (phospho Thr177) antibody validated data

GTX54970 WB Image

Western blot analysis of CaMK1 alpha (pT177) expression in Jurkat Insulin-treated (A) whole cell lysates.

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GTX54970 IHC-P Image

Immunohistochemical analysis of CaMK1 alpha (pT177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w

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GTX54970 ICC/IF Image

Immunofluorescent analysis of CaMK1 alpha (pT177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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GTX54970 WB Image

Western blot analysis of CaMK1 alpha (pT177) expression in Jurkat Insulin-treated (A) whole cell lysates.

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GTX54970 IHC-P Image

Immunohistochemical analysis of CaMK1 alpha (pT177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w

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GTX54970 ICC/IF Image

Immunofluorescent analysis of CaMK1 alpha (pT177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Primary Antibodies