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1 .GTX54970 CaMK1 (phospho Thr177) antibody

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CaMK1 (phospho Thr177) antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX54970
Package Price Qty
100 μlUSD 319.00
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  • Anti-CaMK1 (phospho Thr177) antibody used in Western Blot (WB). GTX54970
  • Anti-CaMK1 (phospho Thr177) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX54970
  • Anti-CaMK1 (phospho Thr177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54970
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  • Datasheet
  • Application Information: CaMK1 (phospho Thr177) antibody

Catalog Number GTX54970
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Product Name CaMK1 (phospho Thr177) antibody
See all CaMKI products
ApplicationsICC/IF, IHC-P, WB
Application NoteWB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Form SuppliedLiquid
PurificationThe antibody was purified by immunogen affinity chromatography.

  • Specifications: CaMK1 (phospho Thr177) antibody

Full Namecalcium/calmodulin-dependent protein kinase I
BackgroundCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]
SynonymsCAMKI Antibody , CAMK1 Antibody , calcium/calmodulin-dependent protein kinase I Antibody
SpecificityRecognizes endogenous levels of CaMK1 alpha (pT177) protein.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human CaMK1 alpha (pT177). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: CaMK1 (phospho Thr177) antibody

Storage BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage InstructionShipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Stem Cell Development > Development > Ectoderm > Nervous system development
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CaMK1 (phospho Thr177) antibody validated data
  • Anti-CaMK1 (phospho Thr177) antibody used in Western Blot (WB). GTX54970
    • GTX54970 WB Image
      Western blot analysis of CaMK1 alpha (pT177) expression in Jurkat Insulin-treated (A) whole cell lysates.
      Top
  • Anti-CaMK1 (phospho Thr177) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX54970
    • GTX54970 IHC-P Image
      Immunohistochemical analysis of CaMK1 alpha (pT177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w
      Top
  • Anti-CaMK1 (phospho Thr177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54970
    • GTX54970 ICC/IF Image
      Immunofluorescent analysis of CaMK1 alpha (pT177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
  • Anti-CaMK1 (phospho Thr177) antibody used in Western Blot (WB). GTX54970
    • GTX54970 WB Image
      Western blot analysis of CaMK1 alpha (pT177) expression in Jurkat Insulin-treated (A) whole cell lysates.
      Top
  • Anti-CaMK1 (phospho Thr177) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX54970
    • GTX54970 IHC-P Image
      Immunohistochemical analysis of CaMK1 alpha (pT177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w
      Top
  • Anti-CaMK1 (phospho Thr177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54970
    • GTX54970 ICC/IF Image
      Immunofluorescent analysis of CaMK1 alpha (pT177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
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