APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.3μg/ml |
| 10μg/ml |
| 3.75μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the membrane of U2OS and U251 cells.
IHC-P
Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88413-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_741960.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium/calmodulin dependent protein kinase II alpha , CAMKA , CaMKIINalpha , MRD53 , MRT63
Cellular Localization
Cell junction, synapse , Cell projection, dendrite
Background
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]
Database
Research Area
DATA IMAGES
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GTX88413 IHC-P Image
IHC-P analysis of human adrenal gland using GTX88413 CaMKII alpha antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88413 WB Image
WB analysis of human cerebellum (A), mouse (B) and rat (C) brain lysate using GTX88413 CaMKII alpha antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88413 FACS Image
FACS analysis of PFA fixed Kelly cells using GTX88413 CaMKII alpha antibody, Internal.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX88413 IHC-P Image
IHC-P analysis of human cortex using GTX88413 CaMKII alpha antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX88413 ICC/IF Image
ICC/IF analysis of PFA fixed U251 cells using GTX88413 CaMKII alpha antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX88413 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX88413 CaMKII alpha antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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REFERENCE
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REVIEW
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