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  • Calcineurin B antibody

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1 .GTX114181 Calcineurin B antibody

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Calcineurin B antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • ★★★★★   (  1  )
Cat No: GTX114181
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
  • Anti-Calcineurin B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114181
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  • Datasheet
  • Star Review (1)
    Reference (0)
  • Bio-info
  • Protocol & MSDS

  • Application Information: Calcineurin B antibody

Catalog Number GTX114181
★★★★★
( 1 )
Publication ( 0 )
Product Name Calcineurin B antibody
See all Calcineurin B products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western blot1:500-1:50000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size19 kDa (note) IsoForm (2)
Positive ControlsNT2D1 , IMR32 , U87-MG , MCF-7 , mouse fetal brain , rat brain , *MEF
Form SuppliedLiquid
Concentration0.76 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Calcineurin B antibody

Full Nameprotein phosphatase 3, regulatory subunit B, alpha
Product DescriptionRabbit Polyclonal antibody to Calcineurin B (protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform)
BackgroundRegulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
SynonymsCALNB1 antibody, CNB antibody, CNB1 antibody, PPP3R1 antibody, protein phosphatase 2B regulatory subunit B alpha antibody, "protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) antibody", "protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) antibody", "protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform antibody", "calcineurin B, type I (19kDa) antibody", calcineurin subunit B type 1 antibody, protein phosphatase 2B regulatory subunit 1 antibody, "protein phosphatase 3, regulatory subunit B, alpha antibody"
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Calcineurin B. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Xenopus laevis, Bovine
Predict Reactivity NoteXenopus laevis (97%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: Calcineurin B antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Cardiovascular > Heart > Heart disease
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Calcineurin B antibody validated data
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000.
      Top
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:50000.
      Top
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Sample (30 ug of whole cell lysate)

      A: IMR32

      12% SDS PAGE

      GTX114181 diluted at 1:1000
      Top
  • Anti-Calcineurin B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114181
    • GTX114181 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B(GTX114181) antibody at 1:250 dilution.
      Top
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000.
      Top
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:50000.
      Top
  • Anti-Calcineurin B antibody used in Western blot. GTX114181
    • GTX114181 WB Image
      Sample (30 ug of whole cell lysate)

      A: IMR32

      12% SDS PAGE

      GTX114181 diluted at 1:1000
      Top
  • Anti-Calcineurin B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114181
    • GTX114181 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B(GTX114181) antibody at 1:250 dilution.
      Top
  • StarReview

  • Calcineurin B antibody

  • Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
  • Western blot(1) :★★★★★
  • Anonymous StarReview submitted on 13 Jun 2014
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Mouse
  • Sample : MEF cells
  • Amount used(ug/cells) : 40ug
  • Blocking conditions
  • Blocking Buffer : 5% non-fat milk
  • Incubation Time & Temperature : 22 (℃)/1hr
  • Primary antibody
  • Dilution : 1:800( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/12hr
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPPP3R15534P63098, B2RC10601302BC027913AAH2791319.3
Homo sapiensPPP3R15534P63098, B2RC10601302NM_000945NP_00093619.3

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusPpp3r119058Q63810NM_024459NP_07777919.3
Rattus norvegicusPpp3r129748P63100NM_017309NP_05900519.3
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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