Calcineurin B antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, IHC-P, WB
Species reactivityHuman, Mouse, Rat
★★★★★ ( 1 )

Cat No:

GTX114181

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: Calcineurin B antibody

Catalog Number

GTX114181

Product Name

Calcineurin B antibody

See all Calcineurin B products

Applications

ICC/IF, IHC-P, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Western Blot (WB)	1:500-1:50000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

19 kDa (note) IsoForm

(2)

Positive Controls

NT2D1 , IMR32 , U87-MG , MCF-7 , mouse fetal brain , rat brain , *MEF

Form Supplied

Liquid

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Calcineurin B antibody

Full Name	protein phosphatase 3, regulatory subunit B, alpha
Background	Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Synonyms	CALNB1 antibody, CNB antibody, CNB1 antibody, PPP3R1 antibody, protein phosphatase 2B regulatory subunit B alpha antibody, "protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) antibody", "protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) antibody", "protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform antibody", "calcineurin B, type I (19kDa) antibody", calcineurin subunit B type 1 antibody, protein phosphatase 2B regulatory subunit 1 antibody, "protein phosphatase 3, regulatory subunit B, alpha antibody"
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human Calcineurin B. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Xenopus laevis, Bovine
Predict Reactivity Note	Xenopus laevis (97%), Bovine (100%)
Conjugation	Unconjugated

Storage Conditions: Calcineurin B antibody

Storage Buffer	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Disease Related > Cardiovascular > Heart > Heart disease More Hide

Calcineurin B antibody validated data

GTX114181 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000.

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GTX114181 ICC/IF Image

Calcineurin B antibody detects Calcineurin B protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcineurin B stained by Calcineurin B antibody (GTX114181) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX114181 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:50000.

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GTX114181 WB Image

Sample (30 ug of whole cell lysate)

A: IMR32

12% SDS PAGE

GTX114181 diluted at 1:1000

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GTX114181 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B(GTX114181) antibody at 1:250 dilution.

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GTX114181 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000.

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GTX114181 ICC/IF Image

Calcineurin B antibody detects Calcineurin B protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcineurin B stained by Calcineurin B antibody (GTX114181) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX114181 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:50000.

Top

GTX114181 WB Image

Sample (30 ug of whole cell lysate)

A: IMR32

12% SDS PAGE

GTX114181 diluted at 1:1000

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GTX114181 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B(GTX114181) antibody at 1:250 dilution.

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StarReview
Calcineurin B antibody
Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
Western Blot (WB)(1) :★★★★★

Anonymous StarReview submitted on 13 Jun 2014
Score : ★★★★★
Application Tested : WB
Sample Species : Mouse
Sample : MEF cells
Amount used(ug/cells) : 40ug
Blocking conditions
Blocking Buffer : 5% non-fat milk
Incubation Time & Temperature : 22 (℃)/1hr
Primary antibody
Dilution : 1:800( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/12hr

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	PPP3R1	5534	P63098, B2RC10	601302	BC027913	AAH27913	19.3
Homo sapiens	PPP3R1	5534	P63098, B2RC10	601302	NM_000945	NP_000936	19.3

Cross-react Bioinfo
Species	Gene name	Gene ID	SwissProt	Isoform (mRNA)	Protein Accession#	MW
Mus musculus	Ppp3r1	19058	Q63810	NM_024459	NP_077779	19.3
Rattus norvegicus	Ppp3r1	29748	P63100	NM_017309	NP_059005	19.3

*MSDS

MSDS-Glycerol.pdf

MSDS-Thimerosal.pdf

Primary Antibodies

Secondary Antibodies

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