Calnexin antibody, Internal (near N-term)
|Applications||ELISA, IHC, IHC-P, WB|
|Application Note||Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1µg/ml. Reported variants represent identical protein: NP_001019820.1, NP_001737.1 |
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Product Description||Goat polyclonal antibody to Calnexin|
|Background||Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. |
|Synonyms||CANX, major histocompatibility complex class I antigen-binding protein p88, calnexin, P90, IP90, CNX, FLJ26570, major histocompatibility complex class I antigenbinding protein p88|
|Cellular Localization||Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
|Specificity||This antibody is specific for Calnexin.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Dog, Pig, Bovine|