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  • Calpain 1 antibody [N3C2], Internal

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1 .GTX102340 Calpain 1 antibody [N3C2], Internal

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Calpain 1 antibody [N3C2], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse, Squirrel
  • Publication  ( 2 )
Cat No: GTX102340
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation. GTX102340
  • Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
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  • Application Information: Calpain 1 antibody [N3C2], Internal

Catalog Number GTX102340
☆☆☆☆☆
( 0 )
Publication ( 2 )
Product Name Calpain 1 antibody [N3C2], Internal
See all Calpain 1 products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size82 kDa (note) IsoForm (4)
Positive Controls293T , A431 , HeLa , HepG2
Form SuppliedLiquid
Concentration0.97 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Calpain 1 antibody [N3C2], Internal

Full Namecalpain 1, (mu/I) large subunit
BackgroundThe calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]
SynonymsCANP antibody, CANP1 antibody, CANPL1 antibody, muCANP antibody, muCL antibody, CAPN1 antibody, calcium-activated neutral proteinase 1 antibody, calpain mu-type antibody, micromolar-calpain antibody, cell proliferation-inducing gene 30 protein antibody, CANP 1 antibody, cell proliferation-inducing protein 30 antibody, "calpain, large polypeptide L1 antibody", calpain-1 catalytic subunit antibody, calpain-1 large subunit antibody, "calpain 1, (mu/I) large subunit antibody"
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Squirrel
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Sheep, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (88%), Pig (96%), Sheep (96%), Rhesus Monkey (98%), Bovine (94%)
ConjugationUnconjugated

  • Storage Conditions: Calpain 1 antibody [N3C2], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Calpain 1 antibody [N3C2], Internal validated data
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
    • GTX102340 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340
    • GTX102340 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation. GTX102340
    • GTX102340 IP Image
      Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
      Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
    • GTX102340 WB Image
      Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
    • GTX102340 IHC-P Image
      Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse lung.
      Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
    • GTX102340 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340
    • GTX102340 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation. GTX102340
    • GTX102340 IP Image
      Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
      Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
    • GTX102340 WB Image
      Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102340
    • GTX102340 IHC-P Image
      Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse lung.
      Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.
      Top
Back to Top
  • Reference for Calpain 1 antibody [N3C2], Internal
Application References Application Reactivity
Yang HH et al. The putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of ketamine cystitis - Revealed by a prospective pilot study using proteomic approaches. Proteomics Clin Appl (Epub ahead of print) (2016). WB Human
Zhang Y et al. Regulation of gene expression by NFAT transcription factors in hibernating ground squirrels is dependent on the cellular environment. Cell Stress Chaperones (Epub ahead of print) (2016). WB Squirrel
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCAPN1823P07384114220NP_00118579975.85
Homo sapiensCAPN1823P07384114220NM_001198868NP_00118579781.9
Homo sapiensCAPN1823P07384114220NM_001198869NP_00118579881.9
Homo sapiensCAPN1823P07384114220NM_005186NP_00517781.9

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusCapn112333O35350, Q3TB79, Q3TI07NM_007600NP_03162682.12
Mus musculusCapn112333O35350, Q3TB79, Q3TI07NM_001110504NP_00110397482.12
Rattus norvegicusCapn129153P97571NM_019152NP_06202582.13
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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