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Catalog Number
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GTX102340
(
2
)
|
|
Product Name
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Calpain 1 antibody [N3C2], Internal
|
| Applications | ICC/IF, IHC-P, IP, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:100-1:1000* |
|
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Immunoprecipitation | 1:100-1:500* |
|
| Western Blot (WB) | 1:500-1:3000* |
Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 82 kDa (note)
 (4)
|
| Positive Controls | 293T , A431 , HeLa , HepG2 |
| Form Supplied | Liquid |
| Concentration | 0.97
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | calpain 1, (mu/I) large subunit |
| Background | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq] |
| Synonyms | CANP antibody, CANP1 antibody, CANPL1 antibody, muCANP antibody, muCL antibody, CAPN1 antibody, calcium-activated neutral proteinase 1 antibody, calpain mu-type antibody, micromolar-calpain antibody, cell proliferation-inducing gene 30 protein antibody, CANP 1 antibody, cell proliferation-inducing protein 30 antibody, "calpain, large polypeptide L1 antibody", calpain-1 catalytic subunit antibody, calpain-1 large subunit antibody, "calpain 1, (mu/I) large subunit antibody" |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Squirrel |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Rat, Pig, Sheep, Rhesus Monkey, Bovine |
| Predict Reactivity Note | Rat (88%), Pig (96%), Sheep (96%), Rhesus Monkey (98%), Bovine (94%) |
| Conjugation | Unconjugated |