Caspase 3 antibody
|Applications||ICC/IF, IHC-Fr, IHC-P, IP, WB|
Not tested in other applications.
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||32 kDa (note)
|Positive Controls||HeLa , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , K562 , THP-1 , HL-60 , Jurkat , Raji , NCI-H929 , HeLa(30 µM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , *PA-1 , *C6|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Product Description||Rabbit Polyclonal antibody to Caspase 3 (caspase 3, apoptosis-related cysteine peptidase)|
|Background||This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]|
|Synonyms||CPP32 antibody, CPP32B antibody, SCA-1 antibody, CASP3 antibody, caspase-3 antibody, apopain antibody, procaspase3 antibody, CASP-3 antibody, protein Yama antibody, "caspase 3, apoptosis-related cysteine protease antibody", CPP-32 antibody, cysteine protease CPP32 antibody, Yama antibody, SREBP cleavage activity 1 antibody, PARP cleavage protease antibody, "caspase 3, apoptosis-related cysteine peptidase antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Cat, Pig, Rabbit, Chimpanzee, Bovine|
|Predict Reactivity Note||Dog (89%), Cat (87%), Pig (88%), Rabbit (86%), Chimpanzee (99%), Bovine (84%)|