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Caspase 3 (cleaved Asp175) antibody

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86909

Cat. No. GTX86909

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

Caspase 3 (cleaved Asp175) antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3 (126-175).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10722560

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CASP3 , CPP32 , CPP32B , SCA1 , caspase 3 , Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86909

GTX86909 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86909 Caspase 3 (cleaved Asp175) antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Ruan Y et al. Mol Med Rep 2015; 12 (4) : 5796-806 Autophagy inhibition enhances isorhamnetin-induced mitochondria-dependent apoptosis in non-small cell lung cancer cells.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399