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1 .GTX123679 Caspase 7 antibody

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Caspase 7 antibody

Knockout/Knockdown
Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman
Cat No: GTX123679
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
  • Anti-Caspase 7 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX123679
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  • Datasheet
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  • Application Information: Caspase 7 antibody

Catalog Number GTX123679
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Product Name Caspase 7 antibody
See all Caspase 7 products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size34 kDa (note) IsoForm (8)
Positive Controls293T , A431 , HeLa , HepG2 , Jurakt , Jurakt (10 μM etoposide treatment for 24 hr)
Form SuppliedLiquid
Concentration0.93 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Caspase 7 antibody

Full Namecaspase 7, apoptosis-related cysteine peptidase
BackgroundThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]
SynonymsCMH-1 antibody, ICE-LAP3 antibody, MCH3 antibody, CASP7 antibody, "caspase 7, apoptosis-related cysteine protease antibody", CASP-7 antibody, Lice2 alpha/beta/gamma antibody, caspase 7 isoform delta antibody, apoptotic protease MCH-3 antibody, caspase-7 antibody, ICE-like apoptotic protease 3 antibody, "caspase 7, apoptosis-related cysteine peptidase antibody"
Cellular LocalizationCytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the C-terminus region of human Caspase 7. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Zebrafish, Xenopus laevis
Predict Reactivity NoteMouse (91%), Rat (91%), Zebrafish (82%), Xenopus laevis (81%)
ConjugationUnconjugated

  • Storage Conditions: Caspase 7 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Metastasis and Invasion > Metastasis > Lymphatic
  • Cancer > Type of cancer > Endometrial
  • Cancer > Type of cancer > Gastrointestinal > Stomach
  • Cancer > Type of cancer > Lung > Other
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Caspase 7 antibody validated data
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Untreated (–) and treated (+) Jurakt whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-Caspase 7 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX123679
    • GTX123679 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
      Top
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
    • GTX123679 WB Image
      Untreated (–) and treated (+) Jurakt whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-Caspase 7 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX123679
    • GTX123679 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NP_00125398534.28
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NP_00125398643.73
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NP_00125398731.62
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NP_00130784035.48
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761BC015799AAH1579934.3
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NM_001227NP_00121834.28
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NM_033338NP_20312437.82
Homo sapiensCASP7840P55210, B5BU45, Q5SVL3601761NM_033339NP_20312534.28

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusCasp712369P97864, Q4FJQ4NM_007611NP_03163734.07
Rattus norvegicusCasp764026O88550NM_022260NP_07159634.33
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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