|Background||Human cell lines (HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1)|
Human Cell Array II carries 6 different human cell lines, including HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1. Cells are harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.