|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||20 kDa (note)
|Positive Controls||A549 , H1299 , HCT-116 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||centrin, EF-hand protein, 3|
|Product Description||Rabbit Polyclonal antibody to Centrin3 (centrin, EF-hand protein, 3)|
|Background||The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. [provided by RefSeq]|
|Synonyms||centrin, EF-hand protein, 3 Antibody, CEN3 Antibody, CETN3 Antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Centrin3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (83%), Xenopus laevis (89%), Rhesus Monkey (100%), Bovine (98%)|