|Full Name||checkpoint kinase 1|
|Product Description||Rabbit Polyclonal antibody to Chk1 (phospho Ser317) (checkpoint kinase 1)|
|Background||Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. |
|Synonyms||Chk1 (phospho Ser317) Antibody , phospho Chk1 (pS317) Antibody , Chk1 phospho Ser317 Antibody , phospho Ser317 Chk1 Antibody , CHEK1 Antibody , Chk1 (pSer317) Antibody , Chk1 (pS317) Antibody , checkpoint kinase 1 Antibody , Chk1 (phospho S317) Antibody |
|Cellular Localization||Nucleus , Cytoplasm|
|Target||Chk1 (phospho Ser317)|
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1 (phospho Ser317). The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Bovine (100%)|