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  • Chk1 antibody [C1C2-3], Internal

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1 .GTX100067 Chk1 antibody [C1C2-3], Internal

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Chk1 antibody [C1C2-3], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman
Cat No: GTX100067
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Chk1 antibody [C1C2-3], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100067
  • Anti-Chk1 antibody [C1C2-3], Internal used in Western Blot (WB). GTX100067
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  • Application Information: Chk1 antibody [C1C2-3], Internal

Catalog Number GTX100067
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Product Name Chk1 antibody [C1C2-3], Internal
See all Chk1 products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size54 kDa (note) IsoForm (6)
Positive ControlsNT2D1 , IMR32
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Chk1 antibody [C1C2-3], Internal

Full NameCHK1 checkpoint homolog (S. pombe)
BackgroundRequired for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
SynonymsCHK1 antibody, CHEK1 antibody, cell cycle checkpoint kinase antibody, "Checkpoint, S. pombe, homolog of, 1 antibody", serine/threonine-protein kinase Chk1 antibody, checkpoint kinase-1 antibody, CHK1 checkpoint homolog antibody, CHK1 checkpoint homolog (S. pombe) antibody
Cellular LocalizationNucleus , Cytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Chk1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Bovine
Predict Reactivity NoteMouse (100%), Rat (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: Chk1 antibody [C1C2-3], Internal

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Brain > Glioma
  • Cancer > Type of cancer > Brain > Meningeal
  • Cancer > Type of cancer > Breast > Other
  • Cancer > Type of cancer > Colorectal > Colon
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Chk1 antibody [C1C2-3], Internal validated data
  • Anti-Chk1 antibody [C1C2-3], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100067
    • GTX100067 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Chk1(GTX100067) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-Chk1 antibody [C1C2-3], Internal used in Western Blot (WB). GTX100067
    • GTX100067 WB Image
      Sample (30 ug of whole cell lysate)
      A: NT2D1
      10% SDS PAGE
      GTX100067 diluted at 1:1000
      Top
  • Anti-Chk1 antibody [C1C2-3], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100067
    • GTX100067 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Chk1(GTX100067) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-Chk1 antibody [C1C2-3], Internal used in Western Blot (WB). GTX100067
    • GTX100067 WB Image
      Sample (30 ug of whole cell lysate)
      A: NT2D1
      10% SDS PAGE
      GTX100067 diluted at 1:1000
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCHEK11111O14757603078NP_00123177550.42
Homo sapiensCHEK11111O14757603078NP_00131735655.53
Homo sapiensCHEK11111O14757603078NP_00131735743.71
Homo sapiensCHEK11111O14757603078NM_001114121NP_00110759354.44
Homo sapiensCHEK11111O14757603078NM_001114122NP_00110759454.44
Homo sapiensCHEK11111O14757603078NM_001274NP_00126554.44

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusChek112649O35280NM_007691NP_03171754.39
Rattus norvegicusChek1140583Q91ZN7NM_080400NP_53632554.44
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.