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Cofilin 1 antibody

KO/KD Orthogonal
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

Cat. No. GTX102156

Reference 19
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cofilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Cofilin 1 antibody detects Cofilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody (GTX102156) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 1 (non-muscle)(GTX102156) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156

GTX102156 IHC-P Image

Cofilin 1 antibody detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody (GTX102156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

REFERENCE

Application Reference

Lee SH et al. Int J Mol Sci 2020; 21 (16) Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.
Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Williams HC et al. Lab Invest 2019; 99 (3) : 399-410 The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo.
Diego Lucero et al. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2019; 1865 (2) : 158537 Interleukin 10 promotes macrophage uptake of HDL and LDL by stimulating fluid-phase endocytosis.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Cofilin 1 antibody Cat. No. GTX102156

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Lee SH et al. Int J Mol Sci 2020; 21 (16) Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.
Chen SY et al. Stem Cells Transl Med 2020; 9 (4):499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Williams HC et al. Lab Invest 2019; 99 (3):399-410 The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo.
Bulletin of the Korean Chemical Society 2019; (Epub) Transgelin Depletion is Critical for the TGFβ1?mediated Initiation of PLCγ1?Cofilin?driven Morphological and Migratory Changes in MDA?MB?231 Cells.
Diego Lucero et al. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2019; 1865 (2):158537 Interleukin 10 promotes macrophage uptake of HDL and LDL by stimulating fluid-phase endocytosis.
Application : WB
Reactivity : Mouse
Yu HI et al. J Pharm Biomed Anal 2018; 160:344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chou HC et al. Life Sci 2018; (Epub) Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647:10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Dettling S et al. Clin Cancer Res 2018; (Epub) Identification of CRKII, CFL1, CNTN1, NME2, and TKT as Novel and Frequent T-cell Targets in Human IDH-Mutant Glioma.
Application : IHC-Fr
Reactivity : Human
Stakaityt? G et al. J Virol 2017; (Epub) Merkel cell polyomavirus small T antigen drives cell motility via Rho-GTPase-induced filopodia formation.
Application : WB
Reactivity : Human
Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
Application : WB
Reactivity : Human
Wang CY et al. CLINICS ONCOL 2016; 1:1015 Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with Down-Regulation of SNAIL-1 and Induction of Let-7.
Application : WB
Reactivity : Human
MarceloF.Montenegro et al. FreeRadicalBiologyandMedicine 2015; 85:288-94 Nox4-dependent activation of cofilin mediates VSMC reorientation in response to cyclic stretching
Application : WB
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta 2015; 1852 (5):851-61 Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Application : WB
Reactivity : Human
Toyo-Oka K et al. J Neurosci 2014; 34 (36):12168-81 14-3-3ε and ζ Regulate Neurogenesis and Differentiation of Neuronal Progenitor Cells in the Developing Brain.
Application : WB
Reactivity : Mouse
Wu CL et al. Electrophoresis 2013; 34 (3):388-96 Hyaluronic acid-dependent protection against alkali-burned human corneal cells.
Application : ICC/IF
Reactivity : Human
Lin LT et al. Mol Imaging Biol 2013; 15 (3):262-72 A comparative study of primary and recurrent human glioblastoma multiforme using the small animal imaging and molecular expressive profiles.
Application : WB
Reactivity : Human
Maheswaranathan M et al. J Biol Chem 2011; 286 (41):35430-7 Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity via Nox1-dependent auto-dephosphorylation of serine 834 in vascular smooth muscle cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169