Collagen II antibody
|Applications||ELISA, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|ELISA||1:5000 - 1:50000*
| ICC/IF||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:50 - 1:400*
|Western blot||1:1000 - 1:10000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||* chondrocyte tissue |
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Purification Note||Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered.|
|Full Name||collagen, type II, alpha 1|
|Product Description||Rabbit Polyclonal antibody to Collagen II|
|Background||Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.|
|Synonyms||ANFH Antibody , COL11A3 Antibody , COL2A1 Antibody , AOM Antibody , SEDC Antibody|
|Immunogen||Collagen Type II from human knee cartilage and bovine nasal cartilage.|
|Species Reactivity||Human, Mouse, Rat, Sheep, Bovine|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Sheep|