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  • Collagen IV antibody [COL-94]

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1 .GTX26311 Collagen IV antibody [COL-94]

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Collagen IV antibody [COL-94]


  • Host / ClonalityMouse Monoclonal
  • Clone NameCOL-94
  • ApplicationsDot, EM, ELISA, ICC/IF, IHC-Fr, IHC-P, WB
  • Species reactivityHuman, Rat
  • Publication  ( 3 )
Cat No: GTX26311
Package Price Qty
100 μlUSD 349.00
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  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
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  • Datasheet
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    Reference (3)
  • Application Information: Collagen IV antibody [COL-94]

Catalog Number GTX26311
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Publication ( 3 )
Product Name Collagen IV antibody [COL-94]
See all Collagen IV products
ApplicationsDot, EM, ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Application Note
Suggested dilutionReference
Dot blotAssay-dependent dilution  
Electron microscopyAssay-dependent dilution  
ELISAAssay-dependent dilution  
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution Y
IHC (Frozen sections)Assay-dependent dilution  
IHC (Formalin-fixed paraffin-embedded sections)1:500*  
Western Blot (WB)Assay-dependent dilution Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Form SuppliedLiquid
PurificationUnpurified

  • Specifications: Collagen IV antibody [COL-94]

Full Namecollagen type IV alpha 1
BackgroundCollagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Synonyms120070, FLJ22259, MGC42377, 1285, P02462, 1284, ATS, 1286, COL4A5, 120130, P08572, COL4A4, 1287, CA54, Collagen IV, COL4A2, CA44, 120131, Q01955, COL4A3, 301050, 120090, 1282, ASLN, COL4A1, P29400, P53420, arresten
SpecificityUsing immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.
HostMouse
ClonalityMonoclonal
Clone NameCOL-94
IsotypeIgG1
Immunogenhuman collagen type IV
Antigen SpeciesHuman
Species ReactivityHuman, Rat
Species Does Not React WithCat, Chicken, Goat, Rabbit, Rat, Sheep
ConjugationUnconjugated

  • Storage Conditions: Collagen IV antibody [COL-94]

Storage BufferAscites, 15 mM sodium azide
Storage InstructionKeep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Angiogenesis
  • Disease Related > Cardiovascular > Angiogenesis
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Stem Cell Development > Stem cells > Mesenchymal Stem Cells
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Collagen IV antibody [COL-94] validated data
  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
    • GTX26311 IHC-P Image
      IHC-P analysis of human tongue tissue using GTX26311 Collagen IV antibody [COL-94] at 1:500.
      Top
  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
    • GTX26311 IHC-P Image
      IHC-P analysis of human placenta sections using GTX26311 Collagen IV[COL-94] antibody at 1:500.
      Top
  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
    • GTX26311 IHC-P Image
      IHC-P analysis of human tongue tissue using GTX26311 Collagen IV antibody [COL-94] at 1:500.
      Top
  • Anti-Collagen IV antibody [COL-94] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26311
    • GTX26311 IHC-P Image
      IHC-P analysis of human placenta sections using GTX26311 Collagen IV[COL-94] antibody at 1:500.
      Top
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  • Reference for Collagen IV antibody [COL-94]
Application References Application Reactivity
Huang YH et al. MicroRNA-29a mitigation of endoplasmic reticulum and autophagy aberrance counteracts in obstructive jaundice-induced fibrosis in mice. Exp Biol Med (Maywood) :1535370217741500 (2017). WB Rat
Schendel SA et al. Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics. Aesthet Surg J (Epub ahead of print) (2017). WB Human
McLane JS et al. Stiffened Extracellular Matrix and Signaling from Stromal Fibroblasts via Osteoprotegerin Regulate Tumor Cell Invasion in a 3-D Tumor in Situ Model. Cancer Microenviron (Epub ahead of print) (2016). ICC/IF Human
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